TAG-1 Component Functions and Benefits

TAG-1 has multiple patents issued and pending. It is a Class 1 general reagent pursuant to 21 CFR 864.4010.

TAG-1 is substantially equivalent to formalin.  Tissue should be stabilized at least 6 hours.

Tissue can then be processed with the same protocols as used for FFPE.

1.  Chaotrope
(sodium thiocyanate)

Degrades nucleases and endonucleases

2.  Chelator (EDTA)

Inactivates Ca and Mg driven enzyme systems – decreasing destruction of cellular contents

3.  Penetrant & metabolic modulator (DMSO)

Drives TAG-1 into tissue and modifies apoptotic cytokines function – slowing cell death

4.  1st Kosmotrope

Maintains cellular integrity and surface antigenic structures by water distribution and metabolism of intracellular and extracellular tissue

5.  Buffer
     (sodium phosphate)

Slows cell death and deformation by maintaining pH and slowing cell lytic activity and release of cell contents into tissue

6.  2nd Kosmotrope
    (α-α trehalose)

Maintains integrity of proteins and antigenic structures

7.   Apoptosis substrate

Maintains cellular apoptotic transition and regulatory control cytokines

8.  Mixing formulation

Optimizes components for genetic expression of tissue sample